| Solution Information | help | |
| Enzyme: | Isoform 2 of Nuclear receptor ROR-alpha (Alpha-2) | |
| inhibitor: | BDBM29616 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: Twelve hundred and twenty nine compounds have been identified as hits during a previously described set of experiments entitled "Primary Cell-based High Throughput Screening assay for activators of the nuclear receptor Steroidogenic Factor 1 (SF-1)". Further information on the primary screen can be found by searching on this website for PubChem AID=522. Among those hits, a subset of compounds showing selectivity towards SF-1 was selected by applying the following criteria: 1) SF1% >70% AND S/A>4 OR 2) 70>SF1 % >50 AND S/A>6 OR(3) SF1% >50 AND RORa% <0 where "SF1%" represents the percentage of activation measured in the SF-1 primary screening (AID=522), "RORa%" the percentage of activation measured in the RORa primary screening (AID=560) and "S/A" the ratio SF1% / RORa%. One hundred and seven compounds selected using criteria stated above were assessed in counterscreen experiments against in 10 point, 1:3 serial dilutions starting at a nominal test concentration o | |
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